The Neuro Probe DC8 cell settling chamber is designed to deposit cells in a monolayer on a glass microscope slide. After the wicking and settling process in the chamber, cells adhere to the slide in a random distribution suitable for differential counting, with excellent cellular morphology.
The chamber consists of two precision-machined acrylic plates, a silicone gasket, and assembly hardware. The lower plate has a recess to fit a 25 x 75mm glass microscope slide. The upper plate has eight through holes, which form 280µL wells when the upper plate is clamped onto the microscope slide in the lower plate. The gasket and a sheet of prepunched filter paper (Stock # DC8WF1) pinched between the plates cause liquid in the cell suspension to wick away as the cells settle onto the slide, leaving the cell sites dry.
For best results microscope slides with enhanced binding properties and a hydrophobic mask around the eight settling sites (Stock # DC8SC) are available. These slides improve cell adherence and also restrict pipetted solutions to individual sites. Different solutions can then be applied to settled cells without crosstalk.
When working with cells of relatively low density, such as lymphocytes, the chamber should be spun in a centrifuge equipped with a microplate carrier. A centrifuge accessory pack (Stock # DC8AP) provides an adapter and counterweight for this purpose.
Accompanying each chamber is a DC8 protocol for its use and care.
|Stock #||Well Area||Well Volume||Slide Recess Dimensions|
|DC8||32mm2||280µL||25 x 75 x 1 mm|
Please contact us if you have any questions.