25x80-Filter-wBox-300x300

Filters for AP48, A3BP48, AA12 and AA10

$216.00$380.00

Polycarbonate Membranes 25 x 80 mm (100 filter box)

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SKU: PFB3 - SCB8. Categories: , .

Product Description

Polycarbonate membranes, 25x80mm, (100 filters)

The AP48, AA12, AA10 and A3BP48 chambers use 25x80mm. Sold in lots of 100.

Pore Size 3µm 5µm 8µm 10µm 12µm
Standard PCTE PFB3 PFB5 PFB8 PFB10 PFB12
PCTE w/PVP PRB3 PRB5 PRB8 PRB10
Cellulose Nitrate SCB3 SCB5 SCB8

Polycarbonate track-etch (PCTE) membranes are the primary type of filters used in our chambers and instrument systems. This is a very thin membrane ranging between 6-10µm in nominal thickness. This membrane type offers excellent uniformity in pore size and density, as well as other benefits such as low extractables, low protein binding, and negligible absorption. These characteristics make it ideally suited for use in chemotaxis assays as well as other high purity screening assays.

PVP Treatment –PCTE membranes are available pre-treated with the wetting agent polyvinylpyrrolidone (PVP) which makes the surface hydrophilic. PVP-treated membranes are used when the media solutions cannot wet out the membrane by themselves. This occurs primarily when working with very small pore sizes or high viscosity media solutions. Certain cell types such as neutrophils do not adhere well to PVP treated membranes and tend to drop off after migrating. While this is problematic when quantifying filters on the membrane, some researchers using the ChemoTx® sytem or the MB-series 96-well chambers and quantifying in the plate find this to be an advantage. Extra-cellular matrix proteins do not adhere well to PVP treated membranes either. If your assay requires such coating, we recommend using PVP-free membranes.

Filters for the AP48, AC48, AA12 and AA10 are also available in cellulose nitrate. These are much thicker (~150 µm) fibrous filters, sometimes referred to as “torturous path” filters. Rather than counting cells that have migrated through the membrane, as is commonly done with PCTE membrane, these filters are clarified with solvents after a predetermined incubation, and a high-resolution light microscope is used to observe how far into the filter the cells have migrated. This is also known as a “leading edge” study. These filters have a recommended shelf life of one year. They are cut to order, so please allow 2-3 days for processing.

Polycarbonate membranes are available in the following pore sizes and corresponding pore densities:

Pore size (+0/-20%) 2µm 3µm 5µm 8µm 10µm 12µm 14µm
Density (pores/mm2)
(tolerance +/- 15%)
2 x 104 2 x 104 4 x 103 1 x 103 1 x 103 1 x 103 5 x 102

 

Many factors other than cell type enter into the pore size selection process. Please see our page on Experiment Design.

Additional Information

Weight 0.1875 lbs
Dimensions 5 x 4 x 1 in
Pore Size

3µm, 5µm, 8µm, 10µm, 12µm

Filter Dimensions

25x80mm

Filter Quantity

100 filter/box

Filter Membranes

Standard PCTE, PCTE with PVP, Cellulose Nitrate