Filters for Blind well and Boyden chambers

$170.00$175.00

Polycarbonate Membranes 13mm-disc (100 filter count)

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SKU: PFA-PRA. Categories: , .

Product Description

Filters for Blind well and Boyden chambers

Please select the appropriate filter type and pore size from the drop down lists above before clicking on ‘Add to Cart.’

Single-well chambers, both blind well and Boyden, use 13mm diameter filters. Sold in lots of 100.

Pore Size 2µm 3µm 5µm 8µm 10µm 12µm
Standard PCTE PFA2 PFA3 PFA5 PFA8 PFA10 PFA12
PCTE w/PVP PRA3 PRA5 PRA8

Please contact us for pricing and availability on other pore sizes

Polycarbonate track-etch (PCTE) membranes are the primary type of filters used in our chambers and instrument systems. This is a very thin membrane ranging between 6-10µm in nominal thickness. This membrane type offers excellent uniformity in pore size and density, as well as other benefits such as low extractables, low protein binding, and negligible absorption. These characteristics make it ideally suited for use in chemotaxis assays as well as other high purity screening assays.

PVP Treatment –PCTE membranes are available pre-treated with the wetting agent polyvinylpyrrolidone (PVP) which makes the surface hydrophilic. PVP-treated membranes are used when the media solutions cannot wet out the membrane by themselves. This occurs primarily when working with very small pore sizes or high viscosity media solutions. Certain cell types such as neutrophils do not adhere well to PVP treated membranes and tend to drop off after migrating. While this is problematic when quantifying filters on the membrane, some researchers using the ChemoTx® sytem or the MB-series 96-well chambers and quantifying in the plate find this to be an advantage. Extra-cellular matrix proteins do not adhere well to PVP treated membranes either. If your assay requires such coating, we recommend using PVP-free membranes.

Polycarbonate membranes are available in the following pore sizes and corresponding pore densities:

Pore size (+0/-20%) 2µm 3µm 5µm 8µm 10 or 12µm
Density (pores/mm2)
(tolerance +/- 15%)
2 x 104 2 x 104 4 x 103 1 x 103 1 x 103

 

Many factors other than cell type enter into the pore size selection process. Please see our page on Experiment Design. Please see the charts below for pore size availability for specific products.

 

Additional Information

Weight 0.125 lbs
Dimensions 5 x 4 x 1 in
Filter Membranes

Standard PCTE, PCTE with PVP

Pore Size

2µm, 3µm, 5µm, 8µm, 10µm, 12µm

Filter Quantity

100 filter/box

Filter Dimensions

13mm diameter