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Accessory Pack for Polycarbonate Filters (P48AP)The accessory pack, Stock# P48AP, contains several items to facilitate handling and staining polycarbonate membranes. It includes a filter wiper that may be mounted on a ring stand, one large and one small filter clamp, and two pairs of curved, stainless steel forceps. The implements are used to wipe non-migrated cells off the top of a filter and while fixing and staining the migrated cells. For more detail on these procedures, see the protocol for staining polycarbonate filters.
These accessories may also be purchased individually. Filter wiper – Stock# P48W
Large clamp – Stock# P48CL – a wide plastic clamp to keep the filter extended widthwise
Small clamp – Stock# P48CS – a stainless steel clamp to act as a weight, keeping the filter extended lengthwise while wiping it or dipping it in fixative and staining solutions
Forceps – Stock# P48F
Filter TemplatesThe template for the AP48 chamber, Stock # P48TM, is a thin piece of plastic with a pattern of holes identical to the pattern of wells in the AP48 . The template is used to help locate the spots of cells on the filter after the filter has been stained. For most assays it is not necessary to use the template since there are usually many cells, and the stain is very visible. But if there is very little migration of cells, or if the stain used is not visible, it can be difficult to distinguish cell sites on the filter from spaces between sites.
The template for the AC48 chamber 48 well chemotaxis chamber, stock # C48TM, is similar to the AP48 template, and functions as outlined above for the AP48.
Filter ClipsSSC4 – these stainless steel clips may be used to to attach filters to microscope slides. They are used primarily while staining cellulose nitrate filters. They may also be used to hold a filter template in alignment with the filter. Sold in sets of 4.
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