In the Neuro Probe AA12 chemotaxis chamber chemoattractants added to the lower wells act on cells in a suspension added to the upper wells. A filter separates the upper and lower wells. After incubation the migrated adherent cells on the filter are stained and counted. In comparison with the more commonly used AP48 chamber, the AA12 has larger volume upper and lower wells and a larger exposed filter area.
The AA12 chamber consists of precision-machined acrylic top and bottom plates, assembly hardware, and a silicone gasket. The plates have 12 wells in two offset rows of six wells each. Each well in the bottom plate is 4.75mm in diameter, holds 150µL, and has a rounded base. Corresponding holes in the top plate form the upper wells when the chamber is assembled. These top wells have a volume of 100µL. The exposed filter area is 18mm2.
A single 25 x 80mm piece of filter membrane is placed between the top and bottom plates, and a gasket is positioned over the filter to create a seal. Narrow raised rims around mating surfaces of top and bottom wells enhance the seal.
Before the migrated cells can be stained and counted, the nonmigrated cells must be wiped off the top side of the filter. An accessory pack (Stock # P48AP) includes instruments for this purpose.
The AA12 is designed for use with polycarbonate filters, although cellulose nitrate filters may also be used.
Each chamber is accompanied by a protocol for its use and care. Filters and accessories for the AA12 are the same as used with the AP48.
|Stock #||Bottom well||Top well||Exposed filter area||Filters used|
|AA12||150µL||100µL||18mm2||25 x 80mm polycarbonate
25 x 80mm cellulose nitrate