Polycarbonate track-etch (PCTE) membranes are the primary type of filters used in our chambers and instrument systems. This is a very thin membrane ranging between 6-10µm in nominal thickness. This membrane type offers excellent uniformity in pore size and density, as well as other benefits such as low extractables, low protein binding, and negligible absorption. These characteristics make it ideally suited for use in chemotaxis assays as well as other high purity screening assays.
To provide a filter suited to 96-well applications, Neuro Probe developed the framed filter, in which filter membrane is bonded to a rigid frame that enables the user to hold the filter by the frame during laboratory procedures.
Framed filters are standard in all our 96-well instruments – the MB-series and A-series reusable 96-well chambers as well as our ChemoTx ® disposable system .
Neuro Probe has developed a variety of filter masks and coatings that make our specially treated framed filters a key component in a number of our products. For example, each of our disposable ChemoTx® system instruments includes a framed filter, and a selection of filter characteristics is available to meet the needs of different research applications. See ChemoTx® system and 96-well filters for more information and prices.
Neuro Probe framed filters should always be used according to the protocol provided with each Neuro Probe instrument. In addition, the following warnings must be observed:
- Do not soak framed filters in strong acid solutions. For applications that require weak acid washes, contact us to discuss the best method for such treatment.
- Do not touch the filter portion of a framed filter with any instruments that might cause contamination.
- Do not touch the filter portion of a framed filter with your fingers; the lipids on your fingers can act as chemotactic factors for many cells.
Failure to Adhere to these Warnings Immediately Voids the Warranty.